Sample preparation

The following sample preparation steps are available in our facility. On request, we also offer users the possibility to perform more elaborate sample preparations within our laboratory on their own.

• 1D and 2D SDS-PAGE (mini gel format) incl. Coomassie staining
• Protein digestion
  • Types
    • In-Gel
    • In-Solution (=gel-free)

      • 8M Urea protocol

      • Urea-FASP protocol

  • Proteases
    • Trypsin
    • LysC
    • Other proteases on request
• Automated sample reduction, alkylation and digestion using an Intavis Digest Pro robot (only gel-based)

• Sample desalting (C18-based)
  • Online using a trapping column within the LC-MS setup
  • Offline
    • small sample amounts =

      self-packed C18-StageTips

    • large sample amounts = solid-phase extraction columns (SepPak,...)

• Dimethyl labeling

  (chemical stabel-isotope labeling) for quantitative proteomics
• Additional sample fractionation steps - small (

  StageTip

  format) or large scale (Offline LC format)
  • SCX
  • SAX
  • high-pH
  • SEC