Core Facility for Mass Spectrometry & Proteomics (CFMS)
The Core Facility for Mass Spectrometry and Proteomics is an infrastructure facility of the Center for Molecular Biology of Heidelberg University (ZMBH). We support the research scientists of the FoF1 / Heidelberg and Mannheim Life Sciences campus with high-performance mass spectrometry aimed at protein analytics and proteomics.
Services provided, additional services subject to request:
- Molecular weight determination of intact proteins
- Protein identification of gel separated proteins
- Identification of interaction partners from Co-IP experiments
- Analysis of proximity labeling (BioID) experiments
- Proteome analysis
Before contact please familiarize yourself with our step-by-step guidelines to ensure a smooth and easy process. Once you know which service to request please contact Marcin Luzarowski to schedule an appointment before submission m.luzarowski@zmbh.uni-heidelberg.de
CFMP User Regulations
- Each facility user is obliged to be familiar and sign CFMP user regulations; these also contain information on pricing (Note: the final price has to be discussed with the head of the CFMP)
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Contributions of the CFMP to the research projects should be appropriately acknowledged as this aids the CFMP’s recognition, thus, easing applications for external funding and the acquisition of new instruments. As scientific contribution can be considered:
- Development of new analytical method
- Help with experimental design
- Generation and/or evaluation of the data
- Manuscript preparation, generation of figures, supplementary data preparation and raw data submission
- Sample processing occurs on a first come first serve basis. Regular services are usually processed within 2-4 weeks; additional services may take up to 8 weeks.
Equipment
Mass spectrometers for liquid chromatography (LC), mass spectrometry (MS), and LC-MS/MS:
- Q-Exactive HF MS (Thermo) coupled with Dionex Ultimate 3000 RSLCnano (Thermo)
- Qtrap 5500 MS (ABSciex) coupled with nanoUPLC Aquity (Waters)
- maXis UHR-TOF (Bruker Daltonics) coupled with UHPLC 1290 Infinity (Agilent)
Acknowledgment
We extend our gratitude to our colleagues at EMBL for engaging discussions on website organization and generously sharing informative content from their website.
