Equipment

• Confocal microscopes (1-4)

• TIRF/ScannerFRAP microscope (5)

• Widefield screening microscope (6)

• Widefield microscopes (7-11)

• Micromanipulation/-injection system (12)

• Stereomicroscopes & macroscopes (13-14)

1. Zeiss LSM 780 confocal microscope

• Microscope stand: Zeiss Axio Observer, inverted microscope
• Laser: 405 nm pulsed diode laser, 30 mW / 458 nm, 488 nm, 514 nm Argon laser, 25 mW / 561 nm DPSS laser, 20 mW / 633 nm HeNe laser, 5 mW
• (pulsed) in Tune laser 490-640 nm, 1.5 mW per wavelength
• Objectives: Plan-APOCHROMAT 20x/0,80 Air / C-APOCHROMAT 40x/1.2 W Korr-UV-VIS-IR / Plan-APOCHROMAT 40x/1.3 Oil / Plan-APOCHROMAT 63x/1.4 Oil / Plan-APOCHROMAT 100x/1.4 Oil
• Software: Zeiss ZEN 2010
• Room -132

2. Leica TCS SP5 confocal microscope

• Microscope stand: Leica DMI 6000 CS Trino, inverted microscope
• Laser: 405 nm diode laser, 50 mW / 458 nm, 5 mW, 76 nm, 5mW, 488 nm, 20 mW, 514 nm, 20 mW Argon laser / 561 nm DPSS laser, 20 mW / 633 nm HeNe laser, 10 mW
• Objectives: HC PL APO 10x/0.40 CS Air / HCX PL APO 20x/0.70 IMM CORR Lbd BL / HCX Plan APO 40x/1.30 Oil CS / HCX PL APO 63x/1.20 W CORR CS / HCX PL APO 63x/1.30 GLYC CORR CS / HCX PL APO 63x/1.40-0.60 Oil Lbd BL
• 2 Hybrid GaAsp detectors (HyD) and 3 PMTs
• Tandem scanning system
• Incubation chamber w/ Temp & CO2 control (LIS)
• Software: Leica Application Suite (LAS)
• AFC (hardware autofocus)
• Room -132

3. Leica DMi8 Spinning Disk microscope

• Microscope stand: Leica DMi8, inverted microscope
• Confocal Scanner Unit: Yokogawa CSU-X1
• Laser: 405 nm, diode laser / 488 nm, 50 mW diode laser / 561 nm, 50 mW diode laser / 640 nm, diode laser
• Objectives: HC PL FLUOTAR 25x/0.8 IMM CORR / HC PL APO 40x/1.3 Oil / HC PL APO 63x/1.40-0.60 Oil / HC PL APO 100x/1.4 Oil CS2
• Cameras: Hamamatsu EMCCD ImagEM Enhanced (C9100-13) / Hamamatsu Orca Flash 4.0 LT (C11440-42U)
• Adaptive Focus Control (AFC)
• Software: MetaMorph Advanced Acquisition
• Incubation chamber XL & Pecon temperature control unit
• Room -132

4. Leica TCS SP8 STED 3X microscope with FALCON FLIM

• Microscope stand: Leica DMi8, inverted microscope
• Laser: White light laser (WLL), 470-670 nm, pulsed
• STED lasers for superresolution: 592 nm, 1.5 W / 660 nm, 1.5 W / 775 nm, >1.5 W
• Objectives: HC PL FLUOTAR 10x/0.30 Air / HC PL APO 20x/0.75 IMM CORR Lbd BL / HCX PL APO 63x/1.40 Oil CS2 / HC PL APO 93x/1.30 GLYC motC STED W / HC PL APO 100x/1.40 STED White Oil
• 2 Hybrod GaAsp detectors (HyD) and 2 PMTs
• gated STED
• 3D STED 3X
• Software: Leica Application Suite X (LASX)
• FALCON for FLIM, FLIM-FRET, TauSTED and Phasor
• Room -132

TIRF/ScannerFRAP widefield microscope:

5. Olympus xcellence TIRF/ScannerFRAP

• Microscope stand: Olympus IX81, inverted microscope
• Olympus cellTIRF 4-Line Motorized TIRF Combiner
• TIRF Lasers: 405 nm, 100 mW / 488 nm, 100 mW / 561 nm, 100 mW / 640 nm, 140 mW
• Olympus cellFRAP module
• ScannerFRAP Laser: 514 nm, 100 mW
• Objectives: UPLSAPO 60x/1.35 Oil DIC / PLAPO 100x/1.45 Oil DIC
• MT 20 illumination system: 150 W Xe or 150 W Hg/Xe arc burner / Fast filter wheel
• Filters: Dualband CFP/YFP sbx HC filter set / Dualband GFP/mCherry sbx ET filter set / Quad DAPI/FITC/Cy3(Texas Red)/Cy5 sbx HC filter set / TIRF Quad
• Cameras: Olympus XM10 / Hamamatsu ImagEM Enhanced (C9100-13)
• Software: Olympus xcellence
• ZDC (hardware autofocus)
• Incubation chamber w/ Temp and CO2 control units
• TMC Linos optical table 63-550
• Room -132

Widefield screening microscope:

6. Olympus ScanR screening/CellSens system

• Microscope stand: Olympus IX81, inverted microscope
• Objectives: UPLSAPO 20x/0.75 Air / UPLSAPO 40x/0.95 Air / UPLFL N 60x/0.90 Air / PLAPO 100x/1.45 Oil
• Lumencor Spectra X illumination system
• Filters for: DAPI / CFP / GFP / YFP / RFP/mCherry / Cy5
• Camera: Hamamatsu Orca Flash 4.0
• Software: Olympus ScanR_2.5.0 & CellSens
• ZDC (hardware autofocus)
• Incubation chamber w/ Pecon temp control
• Room -144

Widefield microscopes:

7. Olympus CellSens microscope

• Microscope stand: Olympus IX81, inverted microscope
• Objectives: UPLSAPO 10x/0.4 Air / UPLSAPO 20x/0.75 Air / UPLSAPO 40x/0.95 Air DIC / UPLSAPO 60x/1.35 Oil / PLAPO 100x/1.45 Oil DIC
• Lumencor Spectra X illumination system
• Filters: Dualband CFP/YFP sbx HC filter set / Dualband GFP/mCherry sbx ET filter set / Quad DAPI/FITC/Cy3(Texas Red)/Cy5 sbx HC filter set / Single band GFP / Single band mCherry
• Camera: Hamamatsu ORCA-R2
• Software: Olympus CellSens
• ZDC (hardware autofocus)
• Room -144

8. Nikon BioStation IM-Q Time Lapse Imaging System

• For glass bottom dishes
• Objectives: 40x (20x, 40x, 80x by switching tube lens), NA 0.8,
• Filters: Ex 438/24, Em 483/32 CFP/Cerulean / Ex 472/30, Em 520/35 GFP / Ex 500/24, Em 542/27 YFP / Ex 562/40, Em 624/40 Alexa568/mCherry/TxRed
• Camera: 1.3-megapixel cooled monochrome camera
• Software: Nikon
• Room -144

9. Applied Precision (GE) PersonalDV

• Objectives: UPlanSApo 10x/0.40 Air / UPlanSApo 20x/0.75 Air / UPlanSApo 40x/0.95 Air / UPlanSApo 20x/0.85 Oil / UApo/340 40x/1.35 Iris Oil / PlanApo N 60x/1.42 Oil
• Filters: Dichroic 1: GFP & RFP / Ex 490/20, Em 528/38 / Ex 555/28, Em 617/73 / Dichroid 2: CFP & YFP & mCherry / Triple „Sedat“ sbxm ET Filterset 
• Camera: Photometrics CoolSNAP HQ2
• Software: Resolve3D/softWoRx
• UltimateFocus (hardware autofocus)
• Environmental Chamber
• TMC optical table 63-534
• Room -144

10. Leica DMRXA, upright microscope

• Objectives: N Plan 5x/0.12 PH0 Air / HC PL Fluotar 10x/0.30 PH1 Air / HC PL Fluotar 20x/0.50 PH2 Air / PL Fluotar 40x/0.70 PH2 Air / PL APO 40x/1.25-0.75 Oil / PL APO 63x/1.0 W CORR / PL APO 100x/1.40-0.7 Oil
• Filters: Ex 340-380, Em LP 425 (DAPI) / Ex BP 450-490, Em LP 515 (FITC) / Ex 470/40, Em BP 525/50 (GFP) / Ex 470/40, Em LP 500 (EGFP) / Ex BP 620/60, Em LP 700/75 (Cy5) / Ex 531-560, Em BP 574-648 (TexasRed) / Ex BP 436/20, Em BP 480/40 (CFP) / Ex BP 560/40, Em BP 645/75 (Cy3)
• Camera: Hamamatsu ORCA-ER (C4742-95-12ER)
• Software: Micro-Manager
• Room -144

11. Zeiss AxioObserver

• Microscope stand: Zeiss Axio Observer Z1
• Objectives: LD-Plan-NEOFLUAR 20x/0.4 Air Ph2 / LD-Plan-NEOFLUAR 40x/0.6 Air Ph2 / LD-Plan-NEOFLUAR 40x/1.3 Oil DIC / Plan-APOCHROMAT 63x/1.4 Oil DIC / Plan-NEOFLUAR 100x/1.3 Ph3
• Filters: Ex BP 340-380, Em LP 425 / Ex BP 450-490, Em BP 515-560 / Ex 530-595, Em LP 615 / Ex 570/50, Em 640/40
• Camera: AxioCam MRm
• Software: AxioVision
• Colibri LED light source
• Definite Focus (hardware autofocus)
• Incubation chamber w/ CO2 ModuleS and TempModuleS
• Room -144

Micromanipulation/-injection system:

12. AIS 2 micromanipulation system

• Microscope stand: Zeiss Axiovert 10, inverted microscope
• Injection system: Luigs & Neumann, Germany
• Injection pressure system: Eppendorf FemtoJet
• Objectives: ACHROSTIGMAT 5x/0.12 Air / ACHROSTIGMAT 10x/0.25 Air / ACHROSTIGMAT 20x/0.30 Air Ph1 / ACHROSTIGMAT 32x/0.40 Air Ph1 / Plan NEOFLUAR 40x/0.75 Air
• Filters: F41-017 HQ-Set EGFP / F41-021 HQ-Set Cy 3,5
• Camera: ImagingSource DMK2002
• Software: AIS 2 control program
• Room -144

Stereomicroscopes & macroscopes:

13. Leica MZ FLIII fluorescence stereomicroscope

• Filters: GFP2, CFP, UV, G(reen) ex.
• Cold light source: Leica KL 1500 LCD
• Fluorescence light source: Leica HXP 120W
• Camera: ProgRes C10plus (optional)
• Software: Micro-Manager
• Room -144

14. Leica M420 macroscope

• Cold light sources: Leica KL 1500 LCD & Zeiss KL 2500 LCD
• Camera: ProgRes C10plus (optional)
• Software: Micro-Manager
• Room -144